Regulatory
Part:BBa_K1461107:Design
Designed by: Shunsuke Sumi Group: iGEM14_UT-Tokyo (2014-09-23)
EGP-2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
EPCAM is expressed in CTC. Therefore, EGP-2 is considered to be a good candidate for a marker.
Source
1. McLaughlin, P. M., Trzpis, M., Kroesen, B. J., Helfrich, W., Terpstra, P., Dokter, W. H., ... & Harmsen, M. C. (2004). Use of the EGP-2/Ep-CAM promoter for targeted expression of heterologous genes in carcinoma derived cell lines. Cancer gene therapy, 11(9), 603-612. 2.Danda, R., Krishnan, G., Ganapathy, K., Krishnan, U. M., Vikas, K., Elchuri, S., ... & Krishnakumar, S. (2013). Targeted Expression of Suicide Gene by Tissue-Specific Promoter and MicroRNA Regulation for Cancer Gene Therapy. PloS one, 8(12), e83398.